Yoko Shoji, Ayako Sanekata, Masao Sato, Katsumi Imaizumi
Laboratory of Nutrition Chemistry, Faculty of Bioresources and Bioenvironmental Sciences, Kyushu University, Fukuoka 812-8581, Japan.
Bioscience, biotechnology, and biochemistry 2003 MayAnti-rat delta6-desaturase serum was produced by immunizing rabbits with the 14 N-terminal amino acids (2-15) of rat delta6-desaturase. The antiserum prevented the enzymatic activity of delta6-desaturase in microsomes. Subsequently, the antiserum was used to demonstrate that gemfibrozil, a ligand for peroxisome proliferator-activated receptor alpha, is involved in activating delta6-desaturase gene expression, thereby elevating the protein level and the activity.
Yoko Shoji, Ayako Sanekata, Masao Sato, Katsumi Imaizumi. Preparation of antiserum against rat delta6-desaturase and its use to evaluate the desaturase protein levels in rats treated with gemfibrozil, a ligand for peroxisome proliferator-activated receptor alpha. Bioscience, biotechnology, and biochemistry. 2003 May;67(5):1177-8
PMID: 12834305
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