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To characterize its enzymic property we cloned and sequenced the gene of Deinococcus radiodurans encoding UV-endonuclease beta, an alternative enzyme to UvrABC repairing damaged DNA. Amino acid substitutions were found in UV-sensitive mutants. The putative amino acid sequence had some similarity with those of eukaryotic UV-endonucleases and with a sequence found in a protein data base of Bacillus subtilis.

Citation

Shigeru Kitayama, Issay Narumi, Tomoo Funayama, Hiroshi Watanabe. Cloning of structural gene of Deinococcus radiodurans UV-endonuclease beta. Bioscience, biotechnology, and biochemistry. 2003 Mar;67(3):613-6

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PMID: 12723611

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