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To develop an HPLC method for determining flavoniod-glycosides in Yixintong pills. Agilent ZORBAX Extend-C18 (4.6 mm x 250 mm, 5 microm) was used with tetrahydrofuran-methanol-acetonitrile-water-acetic acid (30:3:4:120:3) as mobile phase. Detection wavelength was 330 nm. Good linearities of rhamnosylvitexin and vetexin-glucoside were obtained within the range of 0.044-1.78 microg (r = 0.9999) and 0.042-0.85 microg (r = 0.9999); the average recoveries were 100.0% and 99.2%; RSD were 0.92% and 1.8%, respectively. This method can be used for quality control of Yixintong pills.

Citation

Juan Su, Wei-dong Zhang, Yun Zhou, Jing Zhou, Zheng-bing Gu. Determination of flavoniod-glycosides in Yixintong pills by HPLC]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. 2004 Jun;29(6):525-7

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PMID: 15706914

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