Mariko Okazaki, Hataitip Nimitkeatkai, Taku Muramatsu, Hikaru Aoyama, Kotomi Ueno, Masaharu Mizutani, Nobuhiro Hirai, Satoru Kondo, Toshiyuki Ohnishi, Yasushi Todoroki
Department of Applied Biological Chemistry, Faculty of Agriculture, Shizuoka University, Shizuoka 422-8529, Japan.
Bioorganic & medicinal chemistry 2011 Jan 1We developed abscinazole-E1 (Abz-E1), a specific inhibitor of abscisic acid (ABA) 8'-hydroxylase (CYP707A). This inhibitor was designed and synthesized as an enlarged analogue of uniconazole (UNI), a well-known plant growth retardant, which inhibits a gibberellin biosynthetic enzyme (ent-kaurene oxidase, CYP701A) as well as CYP707A. Our results showed that Abz-E1 functions as a potent inhibitor of CYP707A and a poor inhibitor of CYP701A both in vitro and in vivo. Abz-E1 application to plants resulted in improved desiccation tolerance and an increase in endogenous ABA. Copyright © 2010 Elsevier Ltd. All rights reserved.
Mariko Okazaki, Hataitip Nimitkeatkai, Taku Muramatsu, Hikaru Aoyama, Kotomi Ueno, Masaharu Mizutani, Nobuhiro Hirai, Satoru Kondo, Toshiyuki Ohnishi, Yasushi Todoroki. Abscinazole-E1, a novel chemical tool for exploring the role of ABA 8'-hydroxylase CYP707A. Bioorganic & medicinal chemistry. 2011 Jan 1;19(1):406-13
PMID: 21115253
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