Li Lu, Liang Wu, Jun Chen, Xiao-hui Lin, Chao Wan, Qing-nan Li
Guangdong Key Laboratory of Pharmaceutical Bioactive Substances, School of Life Science and Biopharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China.
Nan fang yi ke da xue xue bao = Journal of Southern Medical University 2011 NovTo study the effects of sodium on rat osteoblast function and explore the role of epithelial sodium channel (ENaC) in such effects. The proliferation and differentiation of rat osteoblasts were evaluated following treatment with 1×10(-4) mol/L to 1 mol/L Na(+). The mRNA expressions of the osteogenic genes and ENaC-α gene in the treated cells were assessed using RT-PCR. Within the concentration of 1×10(-4) mol/L to 1 mol/L, Na(+) showed a two-way effect on the osteoblasts: low-concentration Na(+) (1×10(-4) mol/L) significantly promoted osteoblast differen- tiation, while at higher concentrations (0.5 and 1 mol/L), Na(+) produced an opposite effect. Sodium did not significantly affect osteoblast proliferation. Low-concentration Na(+) significantly increased the transcription of Cbfa1, OPN and OC, while high concentrations of Na(+) decreased their transcription. Low-concentration Na(+) also enhanced the mRNA expression of ENaC-α, but high-concentration Na(+) treatment lowered ENaC-α mRNA expression. Na(+) displays a direct dose-related effect on osteoblasts by affecting its differentiation, osteogenic gene expression profile, and ENaC-α gene expression, suggesting the involvement of ENaC in Na(+)-mediated functional modulation of rat osteoblasts.
Li Lu, Liang Wu, Jun Chen, Xiao-hui Lin, Chao Wan, Qing-nan Li. Effects of sodium on rat osteoblast and the role of epithelial sodium channel]. Nan fang yi ke da xue xue bao = Journal of Southern Medical University. 2011 Nov;31(11):1871-4
PMID: 22126768
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