A multiplex RT-PCR for rapid and simultaneous detection of viruses and viroids in chrysanthemum.

A Song, Y You, F Chen, P Li, J Jiang, S Chen

College of Horticulture, Nanjing Agricultural University, Nanjing, China.

Letters in applied microbiology 2013 Jan

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Chrysanthemum plants are subject to serious virus diseases, so detection and identification of virus pathogens is important to prevent the virus spread. A reliable one-step multiplex RT-PCR was developed to simultaneously detect two viruses and two viriods: chrysanthemum virus B, tomato Aspermy virus, chrysanthemum stunt viroid and chrysanthemum chlorotic mottle viroid. In addition, we investigated the detection limit and the efficiency of single and multiplex RT-PCR assays. The results showed that the multiplex RT-PCR assay proved to be as sensitive as the single one. In conclusion, this technique is potentially useful in routine diagnosis of chrysanthemum viruses and viroids. The multiplex RT-PCR assay described in this study is the first report of simultaneous detection of virus and viroid in chrysanthemum, which provides a fast, convenient, cost-saving way to detect the virus and viroid mixed infections in plants. © 2012 The Society for Applied Microbiology.

Citation

A Song, Y You, F Chen, P Li, J Jiang, S Chen. A multiplex RT-PCR for rapid and simultaneous detection of viruses and viroids in chrysanthemum. Letters in applied microbiology. 2013 Jan;56(1):8-13