Katrin Westritschnig, Somdeb BoseDasgupta, Vincent Tchang, Kerstin Siegmund, Jean Pieters
Biozentrum, University of Basel, Klingelbergstrasse 50, CH 4056 Basel, Switzerland.
Molecular immunology 2013 AprCoronin 1, which is a member of the evolutionary conserved coronin protein family that is highly expressed in all leukocytes is involved in the activation of the Ca(2+)/calcineurin signaling pathway following cell surface stimulation in T cells, B cells as well as macrophages. Mice deficient for coronin 1 have strongly reduced peripheral T cell numbers as a result of a lack of pro-survival signals for naïve T cells. Whether or not impaired antigen processing and presentation in the absence of coronin 1 expression contributes to this reduction of T cell numbers is unknown. We here show that coronin 1-deficient bone marrow-derived dendritic cells develop normally, and that wild type and coronin 1-deficient dendritic cells were equally able to induce antigen-specific proliferation of T cells. Furthermore, upon immunization, in vivo proliferation of adoptively transferred antigen-specific T cells was comparable in wild type and coronin 1-deficient mice. Finally, infection of wild type and coronin 1-deficient dendritic cells with an ovalbumin-expressing Listeria monocytogenes strain induced comparable levels of ovalbumin-specific T cells responses. Together these results suggest that coronin 1 is dispensable for antigen processing and presentation by dendritic cells. Copyright © 2012 Elsevier Ltd. All rights reserved.
Katrin Westritschnig, Somdeb BoseDasgupta, Vincent Tchang, Kerstin Siegmund, Jean Pieters. Antigen processing and presentation by dendritic cells is independent of coronin 1. Molecular immunology. 2013 Apr;53(4):379-86
PMID: 23099476
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