Haiqing Wu, Ying Peng, Shaojie Wang, Kai Wang, Xunchen Zhao, Fan Jiang
Department of Pharmacy, Shenyang Pharmaceutical University, 103 Wen-Hua Road, Shenyang 110016, China.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 2012 Dec 12A high performance liquid chromatography-quadrupole time of flight mass spectrometry (HPLC-QTOF-MS) method was employed in investigation of benzbromarone metabolites in rat plasma, urine, feces and bile samples. Meanwhile, the metabolic pathways of benzbromarone in rats were discussed. The identification was achieved on a reversed-phase C(18) column with mobile phase gradient method. The QTOF-MS was operated under full scan of MS or MS/MS in negative mode. The fragments were acquired by raising collision induced dissociation (CID) energy for speculating the structures of parent ions. According to the information from the chromatograms and mass spectra, 17 metabolites were obtained. Among them, the deoxidized phase I metabolites and an array of phase II metabolites-sulfate conjugates detected in the biological samples made the work more significant. Copyright © 2012 Elsevier B.V. All rights reserved.
Haiqing Wu, Ying Peng, Shaojie Wang, Kai Wang, Xunchen Zhao, Fan Jiang. Metabolism studies of benzbromarone in rats by high performance liquid chromatography-quadrupole time of flight mass spectrometry. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences. 2012 Dec 12;911:122-32
PMID: 23217315
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