Hoang Lan Phuong, Ju Qiu, Takashi Kuwahara, Keiichi Fukui, Kayo Yoshiyama, Kazusato Matsugano, Norihiko Terahara, Toshiro Matsui
Department of Bioscience and Biotechnology, Division of Bioresource and Bioenviromental Sciences, Faculty of Agriculture, Graduated School of Kyushu University, 6-10-1 Hakozaki, Fukuoka 812-8581, Japan.
Food chemistry 2013 May 1The aim of this study was to clarify the transport behaviour and mechanism of caffeic acid analogue bearing a sugar-moiety, 6-O-caffeoylsophorose (CS), in Caco-2 cells. The absorption of CS was investigated by its transport across Caco-2 cell monolayers using a high-performance liquid chromatography-time-of-flight-mass spectrometry (LC-TOF-MS). The permeation of CS was concentration-dependent and reached the plateau at >6 mM. The apparent permeability (P(app)) of CS in the apical-to-basolateral direction was 5.4×10(-7) cm/s, while in the reversed direction the P(app) value was significantly reduced (1.9×10(-7) cm/s). CS transport was competitively inhibited by phloretin, an inhibitor of monocarboxylic acid transporter (MCT). Benzoic acid, an MCT substrate, also reduced CS transport. A less significant change of CS transport was observed across Caco-2 cell monolayers pretreated with quercetin, a suppressor of tight-junction. These findings strongly indicate that CS, a caffeic acid analogue bearing sophorose moiety, can be transported across Caco-2 cell monolayers via the MCT pathway. Copyright © 2012 Elsevier Ltd. All rights reserved.
Hoang Lan Phuong, Ju Qiu, Takashi Kuwahara, Keiichi Fukui, Kayo Yoshiyama, Kazusato Matsugano, Norihiko Terahara, Toshiro Matsui. Transepithelial transport of 6-O-caffeoylsophorose across Caco-2 cell monolayers. Food chemistry. 2013 May 1;138(1):101-6
PMID: 23265462
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