Nicolas Cougot, Anne-Elisabeth Molza, Emmanuel Giudice, Annie Cavalier, Daniel Thomas, Reynald Gillet
RNA biology 2013 FebA finely tuned balance of translation, storage and decay of mRNAs (mRNAs) is important for the regulation of gene expression. In eukaryotic cells, this takes place in dynamic cytoplasmic RNA-protein granules termed Processing bodies (P-bodies). In this study, by using immunoelectron tomography, 3D modeling and template matching, we analyze the size and the organization of the polysomes in the vicinity of human P-bodies. Our results show the presence of several polysomes that are compatible with a translational activity around P-bodies. Therefore, movement of mRNAs between polysomes and P-bodies can take place when the two compartments are in close contact. The presence of initiation factors in the proximity of P-bodies also suggests that translation of mRNAs can resume at the periphery of these granules.
Nicolas Cougot, Anne-Elisabeth Molza, Emmanuel Giudice, Annie Cavalier, Daniel Thomas, Reynald Gillet. Structural organization of the polysomes adjacent to mammalian processing bodies (P-bodies). RNA biology. 2013 Feb;10(2):314-20
PMID: 23324601
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