Correlation Engine 2.0
Clear Search sequence regions


Sizes of these terms reflect their relevance to your search.

MicroRNAs (miRNAs) are critical to proliferation, differentiation, and development. Here, we characterize gene expression in murine Dicer-null adult mesenchymal stem cell lines, a fibroblast cell type. Loss of Dicer leads to derepression of let-7 targets at levels that exceed 10-fold to 100-fold with increases in transcription. Direct and indirect targets of this miRNA belong to a mid-gestation embryonic program that encompasses known oncofetal genes as well as oncogenes not previously associated with an embryonic state. Surprisingly, this mid-gestation program represents a distinct period that occurs between the pluripotent state of the inner cell mass at embryonic day 3.5 (E3.5) and the induction of let-7 upon differentiation at E10.5. Within this mid-gestation program, we characterize the let-7 target Nr6a1, an embryonic transcriptional repressor that regulates gene expression in adult fibroblasts following miRNA loss. In total, let-7 is required for the continual suppression of embryonic gene expression in adult cells, a mechanism that may underlie its tumor-suppressive function.

Citation

Allan M Gurtan, Arvind Ravi, Peter B Rahl, Andrew D Bosson, Courtney K JnBaptiste, Arjun Bhutkar, Charles A Whittaker, Richard A Young, Phillip A Sharp. Let-7 represses Nr6a1 and a mid-gestation developmental program in adult fibroblasts. Genes & development. 2013 Apr 15;27(8):941-54

Expand section icon Mesh Tags

Expand section icon Substances


PMID: 23630078

View Full Text