Correlation Engine 2.0
Clear Search sequence regions


  • acid (2)
  • AP 2 (3)
  • brain (1)
  • chick embryos (2)
  • chickens (1)
  • endo (1)
  • gene (1)
  • hydrolases (2)
  • NCAM (1)
  • psa ncam (1)
  • retina (7)
  • sialic acids (2)
  • signal (1)
  • TFAP2D (1)
  • Sizes of these terms reflect their relevance to your search.

    Retinal ganglion cells transmit the visual signal from the retina to the brain. We have previously shown that the activator protein 2 (AP-2)δ (TFAP2D) transcription factor is expressed in one third of ganglion cells in developing retina suggesting a specialized role for these AP-2δ-expressing cells. Here, we address the role of AP-2δ in retina by in ovo electroporation of RCAS/AP-2δ retroviral constructs into the eyes of chick embryos at day 2 of gestation. Ectopic expression of AP-2δ does not affect lineage differentiation in the developing retina. However, immunostaining of retinal tissue with markers associated with axonal growth such as growth-associated protein 43 and polysialic acid-neural cell adhesion molecule (PSA-NCAM) demonstrates axonal misrouting and abnormal axonal bundling. Treatment of AP-2δ-misexpressing retinal cell cultures with endoneuraminidase, an enzyme that removes PSA from NCAM, decreases AP-2δ-induced axonal bundling. Our data suggest a role for AP-2δ in polysialylation of NCAM, with ectopic expression of AP-2δ resulting in premature bundling of emerging axons and misrouting of axons. We propose that expression of AP-2δ in a subset of ganglion cells contributes to the fine-tuning of axonal growth in the developing retina. © 2013 International Society for Neurochemistry.

    Citation

    Xiaodong Li, Elizabeth A Monckton, Roseline Godbout. Ectopic expression of transcription factor AP-2δ in developing retina: effect on PSA-NCAM and axon routing. Journal of neurochemistry. 2014 Apr;129(1):72-84

    Expand section icon Mesh Tags

    Expand section icon Substances


    PMID: 24188130

    View Full Text