Correlation Engine 2.0
Clear Search sequence regions


  • cell cycle (1)
  • cells (2)
  • cellular (1)
  • culture media (2)
  • human cells (1)
  • nutrient (3)
  • protein kinases (2)
  • Rim15 (9)
  • signals (1)
  • yeast (2)
  • Sizes of these terms reflect their relevance to your search.

    The PAS kinase Rim15 is proposed to integrate signals from different nutrient-sensing pathways and to control transcriptional reprogramming of Saccharomyces cerevisiae upon nutrient depletion. Despite this proposed role, previous transcriptome analyses of rim15 mutants solely focused on growing cultures. In the present work, retentostat cultivation enabled analysis of the role of Rim15 under severely calorie-restricted, virtually non-growing conditions. Under these conditions, deletion of RIM15 affected transcription of over 10-fold more genes than in growing cultures. Transcriptional responses, metabolic rates and cellular morphology indicated a key role of Rim15 in controlled cell-cycle arrest upon nutrient depletion. Moreover, deletion of rim15 reduced heat-shock tolerance in non-growing, but not in growing cultures. The failure of rim15 cells to adapt to calorie restriction by entering a robust post-mitotic state resembles cancer cell physiology and shows that retentostat cultivation of yeast strains can provide relevant models for healthy post-mitotic and transformed human cells. Copyright © 2014 The Authors. Published by Elsevier B.V. All rights reserved.

    Citation

    Markus M M Bisschops, Priscilla Zwartjens, Sebastiaan G F Keuter, Jack T Pronk, Pascale Daran-Lapujade. To divide or not to divide: a key role of Rim15 in calorie-restricted yeast cultures. Biochimica et biophysica acta. 2014 May;1843(5):1020-30

    Expand section icon Mesh Tags

    Expand section icon Substances


    PMID: 24487068

    View Full Text