Correlation Engine 2.0
Clear Search sequence regions


  • ch TOG (6)
  • chromatin (2)
  • cluster analysis (1)
  • Dyneins (2)
  • gene (3)
  • HSET (2)
  • human cells (1)
  • humans (1)
  • kif11 protein, human (1)
  • kinesin (2)
  • mitosis (1)
  • protein human (3)
  • rna (3)
  • tubulin (2)
  • Sizes of these terms reflect their relevance to your search.

    How multiple spindle assembly pathways are integrated to drive bipolar spindle assembly is poorly understood. We performed an image-based double RNAi screen to identify genes encoding Microtubule-Associated Proteins (MAPs) that interact with the highly conserved ch-TOG gene to regulate bipolar spindle assembly in human cells. We identified a ch-TOG centred network of genetic interactions which promotes centrosome-mediated microtubule polymerisation, leading to the incorporation of microtubules polymerised by all pathways into a bipolar structure [corrected]. Our genetic screen also reveals that ch-TOG maintains a dynamic microtubule population, in part, through modulating HSET activity. ch-TOG ensures that spindle assembly is robust to perturbation but sufficiently dynamic such that spindles can explore a diverse shape space in search of structures that can align chromosomes.

    Citation

    Alexis R Barr, Chris Bakal. A sensitised RNAi screen reveals a ch-TOG genetic interaction network required for spindle assembly. Scientific reports. 2015 Jun 03;5:10564

    Expand section icon Mesh Tags

    Expand section icon Substances


    PMID: 26037491

    View Full Text