A van Waarde, A Lombarts, G van den Thillart, C Erkelens, J Lugtenburg
Department of Animal Physiology, Gorlaeus Laboratories, University of Leiden, The Netherlands.
Haematologia 1989We used 31P Nuclear Magnetic Resonance (NMR) spectroscopy to investigate whether regeneration of ATP in outdated human blood is accompanied by Mg-ATP recomplexation. We found that the loss of 'total ATP' (i.e. the sum of ATP4- + MG-ATP2-) which occurs during blood storage is completely reversible by treatment of RBC with precursors for purine nucleotide salvage. The magnesium-ATP complex, however, is only partially (70%) recovered due to a continuous decline of cytosolic free Mg2+ with increasing RBC age. Total RBC magnesium, as measured by flame absorption spectroscopy, did not show any significant change under these conditions.
A van Waarde, A Lombarts, G van den Thillart, C Erkelens, J Lugtenburg. 31P NMR studies on rejuvenation of outdated red blood cells: complete regeneration of ATP is accompanied by partial Mg-ATP recomplexation. Haematologia. 1989;22(2):79-87
PMID: 2744640
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