Correlation Engine 2.0
Clear Search sequence regions


  • cells (1)
  • ERK (4)
  • mass (2)
  • native (1)
  • protein complexes (2)
  • proteins drosophila (1)
  • SBP (1)
  • sbp tag (2)
  • Sizes of these terms reflect their relevance to your search.

    Elucidation of biological functions of signaling proteins is facilitated by studying their protein-protein interaction networks. Affinity purification combined with mass spectrometry (AP-MS) has become a favorite method to study protein complexes. Here we describe a procedure for single-step purification of ERK (Rolled) and associated proteins from Drosophila cultured cells. The use of the streptavidin-binding peptide (SBP) tag allows for a highly efficient isolation of native ERK signaling complexes, which are suitable for subsequent analysis by mass spectrometry. Our analysis of the ERK interactome has identified both known and novel signaling components. This method can be easily adapted for SBP-based purification of protein complexes in any expression system.

    Citation

    Liu Yang, Alexey Veraksa. Single-Step Affinity Purification of ERK Signaling Complexes Using the Streptavidin-Binding Peptide (SBP) Tag. Methods in molecular biology (Clifton, N.J.). 2017;1487:113-126


    PMID: 27924562

    View Full Text