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Gene-centered yeast one-hybrid (Y1H) screens provide a powerful and effective strategy to identify transcription factor (TF)-promoter interactions. While genome-wide TF ORFeome clone collections are increasingly available, screening protocols have limitations inherent to the properties of the enzymatic reaction used to identify interactions and to the procedure required to perform the assay in a high-throughput format. Here, we present the development and validation of a streamlined strategy for quantitative and fully automated gene-centered Y1H screens using a novel cell surface Gaussia luciferase reporter. © The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

Citation

Katia Bonaldi, Zheng Li, S Earl Kang, Ghislain Breton, Jose L Pruneda-Paz. Novel cell surface luciferase reporter for high-throughput yeast one-hybrid screens. Nucleic acids research. 2017 Oct 13;45(18):e157

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PMID: 28985361

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