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Yeast two-hybrid allows for investigation of interactions between two proteins in vivo. Yeast media can be supplemented with phycocyanobilin, allowing the formation of photoactive phytochromes, and the investigation of their light-regulated interactions. Three reporter assays are described; the histidine growth selection assay for the HIS3 reporter, and the filter lift and quantitative ONPG assays for the LacZ reporter. Design considerations for yeast two- and three-hybrid experiments are also covered.

Citation

David J Sheerin. Investigation of Light-Regulated Protein-Protein Interactions Using Yeast Two-Hybrid Assays. Methods in molecular biology (Clifton, N.J.). 2019;2026:1-19

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PMID: 31317399

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