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The identification of autoantibodies in central nervous system (CNS) inflammatory disorders improves diagnostic accuracy and the identification of patients with a relapsing disease. Usual methods to detect autoantibodies are usually divided into 3 categories: tissue-based assays, protein-based assays and cell-based assays (CBA). Tissue-based assays are commonly used for initial identification of autoantibodies based on staining patterns and co-localization. Once the antigen is known, autoantibodies can be detected using other antigen-specific methods based on recombinant proteins and CBA using transfected cells expressing the protein in their cell membranes. Compared to traditional methods using recombinant proteins such as ELISA and western blot, the CBA have advantage of detecting conformational sensitive antibodies using natively folded proteins in the cell membrane. This article reviews the utility of CBA into the clinical practice. Copyright © 2019 Elsevier B.V. All rights reserved.

Citation

Rachel Dias Molina, Lucas Piccoli Conzatti, Ana Paula Bornes da Silva, Leise Daniele Sckenal Goi, Bruna Klein da Costa, Denise Cantarelli Machado, Douglas Kazutoshi Sato. Detection of autoantibodies in central nervous system inflammatory disorders: Clinical application of cell-based assays. Multiple sclerosis and related disorders. 2020 Feb;38:101858

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PMID: 31775115

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