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    Alternaria fungi are widely distributed plant pathogens infecting grains and vegetables and causing major harvest losses in the field and during postharvest storage. Besides, consumers are endangered by the formation of toxic secondary metabolites. Some of these secondary metabolites are chemically characterized as mycotoxins, but the majority of the Alternaria mycobolome still remains unknown. Fourier-transform ion cyclotron resonance mass spectrometry (FTICR-MS) and LC-MS/MS are combined for the non-targeted and targeted analysis of the metabolome of three A. alternata isolates and one A. solani isolate. Due to the ultra-high resolution of FTICR-MS, unique molecular formulae are assigned to measured m/z signals. The molecular formulae are matched to entries of the databases Antibase and Kyoto Encyclopedia of Genes and Genomes. The non-targeted analysis of the fungal extracts reveals variations in the secondary metabolite profile of A. alternata and A. solani. Differences in the biosynthesis of dibenzo-α-pyrones, perylene quinones, tentoxin, and tenuazonic acid of the A. alternata and A. solani isolates are determined applying targeted LC-MS/MS. FTICR-MS analyses reveal clear differences in the metabolic profile of the A. solani and the A. alternata isolates. © 2019 The Authors. Published by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

    Citation

    Marina Gotthardt, Basem Kanawati, Frank Schmidt, Stefan Asam, Richard Hammerl, Oliver Frank, Thomas Hofmann, Philippe Schmitt-Kopplin, Michael Rychlik. Comprehensive Analysis of the Alternaria Mycobolome Using Mass Spectrometry Based Metabolomics. Molecular nutrition & food research. 2020 Feb;64(3):e1900558

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    PMID: 31802615

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