Correlation Engine 2.0
Clear Search sequence regions


  • carp (7)
  • cross reaction (1)
  • diagnosis (2)
  • dna primers (2)
  • rna (1)
  • rt pcr (1)
  • study time (1)
  • viremia (7)
  • Sizes of these terms reflect their relevance to your search.

    Spring viremia of carp virus (SVCV) is a significant pathogenic agent that can cause large-scale outbreaks of spring viremia of carp (SVC) in many types of fish and bring huge economic losses to the aquaculture industry. A simple and convenient detection method is imperative for SVCV diagnosis. In this study, the real-time reverse transcription recombinase polymerase amplification (RT-RPA) assay was developed and validated. Primers and probe targeting the conserved region of M gene were designed and applied to the real-time RT-RPA assay that performed at 39 °C for 20 min. The specificity analysis showed that no cross-reaction with other pathogenic viruses of fish was found, indicating appropriate specificity of the assay. In vitro transcribed RNA standards were used to estimate the sensitivity of the assay and the detection limit was 102copies/reaction. To further evaluate the assay, 65 clinical samples were tested using both real-time RT-RPA assay and real-time RT-PCR method. The same detection results were observed, suggesting the potential application of real-time RT-RPA assay in clinical sample detection. This is the first report on RPA assay for SVCV detection and this new developed assay would be useful in both laboratory and in the field for diagnosis of SVCV. Copyright © 2019. Published by Elsevier Ltd.

    Citation

    Feng Cong, Fanwen Zeng, Miaoli Wu, Jingjing Wang, Bihong Huang, Yingying Wang, Qing Wang, Shouquan Zhang, Lei Ma, Pengju Guo, Weiwei Zeng. Development of a real-time reverse transcription recombinase polymerase amplification assay for rapid detection of spring viremia of carp virus. Molecular and cellular probes. 2020 Apr;50:101494

    Expand section icon Mesh Tags

    Expand section icon Substances


    PMID: 31863825

    View Full Text