Correlation Engine 2.0
Clear Search sequence regions


Sizes of these terms reflect their relevance to your search.

To develop an iPSC SOX9 reporter line for monitoring differentiation into SOX9 expressing cells such as chondrocytes, cranial neural crest and Sertoli cells, we used gene editing to introduce sequences encoding the tdTomato fluorescent protein into the SOX9 locus. The gene-edited line had a normal karyotype, expressed pluripotency markers and differentiated into cells representative of the three embryonic germ layers. Endogenous SOX9 expression was undisturbed and the tdTomato fluorescent reporter mirrored SOX9 mRNA expression. This iPSC line will be useful for assessing iPSC differentiation into SOX9-expressing cells and enrichment by cell sorting. Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.

Citation

Yudha Nur Patria, Jinia Lilianty, Andrew G Elefanty, Edouard G Stanley, Tanya Labonne, John F Bateman, Shireen R Lamandé. Generation of a SOX9-tdTomato reporter human iPSC line, MCRIi001-A-2, using CRISPR/Cas9 editing. Stem cell research. 2020 Jan;42:101689

Expand section icon Mesh Tags

Expand section icon Substances


PMID: 31884373

View Full Text