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To evaluate and compare the mechanical resistance to tearing of the anterior lens capsule opening after staining with different concentrations of trypan blue in ex vivo porcine eyes. Semmelweis University, and Budapest University of Technology and Economics, Budapest, Hungary. Experimental study. The study comprised 75 porcine eyes. The capsule was unstained in the Control Group (n = 25 eyes), 0.06% trypan blue was used to stain the capsule in Stained 1 Group (n = 25 eyes), and 0.1% trypan blue was used to stain the capsule in Stained 2 Group (n = 25 eyes). After capsulorhexis, the capsule openings were stretched with custom-designed testing equipment until they ruptured. The rupture force (RF), circumference stretching ratio (CSR), and secant modulus at 10 mN (SM10mN) and 50 mN (SM50mN) were evaluated. In total, 75 eyes were enrolled. There were no statistically significant differences in the RF (P = .8924) or CSR (P = .3876) among the groups. There were no statistically significant differences in the SM10mN (P = .8215) or SM50mN (P = .4184) among the groups. In this porcine eye model, the trypan blue concentrations that are routinely used in cataract surgery had no effect on capsular rim stability.

Citation

Gábor L Sándor, Zoltán Kiss, Zoltán I Bocskai, Gábor Tóth, Tamás Temesi, Zoltán Z Nagy. Evaluation of mechanical resistance to tearing of the anterior lens capsule after staining with different concentrations of trypan blue. Journal of cataract and refractive surgery. 2020 Feb;46(2):293-297

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PMID: 32126044

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