Yajie Chi, Zi Liang, Yanwu Guo, Daliang Chen, Lenian Lu, Jiye Lin, Shengcong Qiu, Xiang Wang, Erning Qiu, Famu Lin, Jianmin Chen, Shi Luo, Dahai Zheng, Xiaobing Xu
Brain research bulletin 2020 AugHuman WBSCR22 is involved in cancer proliferation, invasion and metastasis; however, its function in glioma remains unexplored. In our research, we aimed to investigate the role of WBSCR22 in the development of glioma and its possible molecular mechanisms. Using bioinformatic analysis of public datasets, we determined that WBSCR22 overexpression in glioma specimens was correlated with an unfavorable patient prognosis. Our results revealed that WBSCR22 was highly expressed in glioma cell lines. The loss of WBSCR22 inhibited the growth, invasion and migration of glioma cells, while WBSCR22 overexpression produced the opposite effects. Moreover, we found that WBSCR22 downregulation reduced the phosphorylation of Akt and GSK3β and decreased the levels of β-catenin and CyclinD1 in glioma cells. The opposite effects were observed when WBSCR22 was overexpressed. Additionally, we verified with a dual-luciferase reporter assay that WBSCR22 was a direct target of miR-146b-5p. Furthermore, overexpression of miR-146b-5p suppressed WBSCR22 mRNA and protein expression. Notably, the restoration of WBSCR22 expression remarkably reversed the effects of miR-146b-5p overexpression on cell survival, apoptosis and the cell cycle in glioma cells. Collectively, our findings revealed a tumor-promoting role for WBSCR22 in glioma cells, thus providing molecular evidence for WBSCR22 as a novel therapeutic target in glioma. Copyright © 2020 Elsevier Inc. All rights reserved.
Yajie Chi, Zi Liang, Yanwu Guo, Daliang Chen, Lenian Lu, Jiye Lin, Shengcong Qiu, Xiang Wang, Erning Qiu, Famu Lin, Jianmin Chen, Shi Luo, Dahai Zheng, Xiaobing Xu. WBSCR22 confers cell survival and predicts poor prognosis in glioma. Brain research bulletin. 2020 Aug;161:1-12
PMID: 32380188
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