Correlation Engine 2.0
Clear Search sequence regions


  • chromatin (1)
  • embryos (1)
  • gene (3)
  • humans (1)
  • mice (2)
  • stem cells (1)
  • valproic acid (5)
  • Sizes of these terms reflect their relevance to your search.

    The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 system has emerged as a powerful technology, with the potential to generate transgenic animals. Particularly, efficient and precise genetic editing with CRISPR/Cas9 offers immense prospects in various biotechnological applications. Here, we report that the histone deacetylase inhibitor valproic acid (VPA) significantly increases the efficiency of CRISPR/Cas9-mediated gene editing in mouse embryonic stem cells and embryos. This effect may be caused through globally enhanced chromatin accessibility, as indicate by histone hyperacetylation. Taken together, our results suggest that VPA can be used to increase the efficacy of CRISPR/Cas9 in generating transgenic systems.

    Citation

    Hanseul Park, Jaein Shin, Hwan Choi, Byounggook Cho, Jongpil Kim. Valproic Acid Significantly Improves CRISPR/Cas9-Mediated Gene Editing. Cells. 2020 Jun 10;9(6)

    Expand section icon Mesh Tags

    Expand section icon Substances


    PMID: 32532133

    View Full Text