Correlation Engine 2.0
Clear Search sequence regions


  • antibodies (1)
  • blood (2)
  • blood flow (1)
  • botswana (2)
  • brucella (1)
  • brucellosis (4)
  • diagnosis (2)
  • rose bengal (8)
  • serum (1)
  • spp (1)
  • Sizes of these terms reflect their relevance to your search.

    This study was conducted to evaluate the sensitivity (Se) and specificity (Sp) of the Rose Bengal test (RBT), complement fixation test (CFT), the serum lateral flow assay (LFAserum) and the blood lateral flow assay (LFAblood) for the detection of antibodies to Brucella spp. using Bayesian latent class models (BLCMs). Sera and whole blood were collected from naturally infected cattle reared in smallholder, small-scale commercial and large-scale commercial farms in Zimbabwe (n = 1022) and Botswana (n = 770). The BLCMs were fitted under the assumption that conditional dependences existed between the tests. Based on the conditional dependence model, the RBT had the highest Se of 0.897 (95 % Probability Intervals: 0.854; 0.932) compared to 0.827 (0.773; 0.872), 0.812 (0.76; 0.858) and 0.809 (0.785; 0.832) for the LFAserum, LFAblood and CFT, respectively. The CFT recorded a higher Sp of 0.999 (0.995; 1.000) than the LFAserum 0.996 (0.99; 1.000), the LFAblood 0.984 (0.976; 0.991) and the RBT 0.969 (0.959; 0.978). The data indicated that both the Se and Sp of RBT and CFT and the Sp of LFAserum and LFAblood were conditionally independent, while the Se appeared to be conditionally dependent. These results indicated that none of the evaluated tests had perfect Se and Sp and consequently could not be used alone for the diagnosis of brucellosis in cattle from the studied farming sectors. Thus, based on high Se and Sp, respectively, a brucellosis testing regimen using the RBT (screening) and the LFA (confirmatory) may be considered. Copyright © 2020 Elsevier B.V. All rights reserved.

    Citation

    Davies M Pfukenyi, Eleftherios Meletis, Boitumelo Modise, Masimba Ndengu, Faith W Kadzviti, Kopano Dipuo, Kago Moesi, Polychronis Kostoulas, Gift Matope. Evaluation of the sensitivity and specificity of the lateral flow assay, Rose Bengal test and the complement fixation test for the diagnosis of brucellosis in cattle using Bayesian latent class analysis. Preventive veterinary medicine. 2020 Aug;181:105075

    Expand section icon Mesh Tags

    Expand section icon Substances


    PMID: 32622242

    View Full Text