Correlation Engine 2.0
Clear Search sequence regions


filter terms:
Sizes of these terms reflect their relevance to your search.

Optical methods offer the potential to manipulate living biological systems with exceptional spatial and temporal control. Caging bioactive molecules with photocleavable functional groups is an important strategy that could be applied to a range of problems, including the targeted delivery of otherwise toxic therapeutics. However existing approaches that require UV or blue light are difficult to apply in organismal settings due to issues of tissue penetration and light toxicity. Photocaging groups built on the heptamethine cyanine scaffold enable the targeted delivery of bioactive molecules using near-IR light (up to 780nm) in live animal settings. Here we provide a detailed procedure demonstrating the utility of the heptamethine cyanine caging group to create a light-cleavable linker between an antibody, panitumumab, and a therapeutic small molecule in the duocarmycin class of natural products. Descriptions of the design and synthesis of the small molecule component, assembly of the antibody conjugate, in vitro analysis of uncaging, in vivo imaging, and impact on tumor progression are provided. © 2020 Elsevier Inc. All rights reserved.

Citation

Michael P Luciano, Saghar Nourian, Alexander P Gorka, Roger R Nani, Tadanobu Nagaya, Hisataka Kobayashi, Martin J Schnermann. A near-infrared light-mediated cleavable linker strategy using the heptamethine cyanine chromophore. Methods in enzymology. 2020;641:245-275

Expand section icon Mesh Tags

Expand section icon Substances


PMID: 32713525

View Full Text