Correlation Engine 2.0
Clear Search sequence regions


  • 18S rRNA (2)
  • cervid (1)
  • india (2)
  • pcr (1)
  • rna (2)
  • rrna gene (2)
  • sarcocystis (7)
  • sarcocystosis (2)
  • sequence analysis (1)
  • sister (2)
  • spp (3)
  • swine (2)
  • taxa (1)
  • Sizes of these terms reflect their relevance to your search.

    A total of 57 tissue samples of domestic pigs (Sus scrofa) were collected from the meat outlets of five north Indian states and examined for sarcocystosis by histological and molecular methods. The genomic DNA extracted from five representative positive isolates was subjected to PCR amplification of the partial 18S rRNA gene followed by cloning and sequencing. Sequence analysis of the newly generated Indian isolates recorded 96.9-100.0% identity with published sequences of Sarcocystis suihominis. Two new haplotypes that have not been previously described manifested 99.5-100.0% nucleotide homology within themselves. In the phylogenetic analysis, Indian isolates of S. suihominis grouped together with S. suihominis originating from Italy, and they collectively formed a sister clade with Sarcocystis miescheriana within a clade containing various Sarcocystis spp. of ruminants having felids as final hosts. At the same time, this clade separated from a sister clade containing Sarcocystis spp. of bovid or cervid ruminants using canids as known or surmised definitive host. The current study established the phylogenetic relationship of Indian isolates of S. suihominis with various Sarcocystis spp. as well as with other taxa of Sarcocystidae family based on 18S rRNA gene for the first time.

    Citation

    Ravi Pratap Chauhan, Ansu Kumari, Anil Kumar Nehra, Hira Ram, Rajat Garg, P S Banerjee, M Karikalan, A K Sharma. Genetic characterization and phylogenetic analysis of Sarcocystis suihominis infecting domestic pigs (Sus scrofa) in India. Parasitology research. 2020 Oct;119(10):3347-3357

    Expand section icon Mesh Tags

    Expand section icon Substances


    PMID: 32833051

    View Full Text