Evaluation of the nutrition and determination of phosphoproteins is of great importance in different foods as aberrant phosphorylation changes many biological processes and can relate to health conditions. In this study, an ultrafast (5 min) and sensitive electrochemiluminescence (ECL) sensor was innovatively fabricated for the determination of phosphoproteins in foods on the basis of fluorescent probe NH2-TiO2/upconversion nanomaterials (UCNPs). Impressively, the ECL intensity of NH2-TiO2/UCNPs-rGO/GCE was remarkably enhanced by 29 times. Furthermore, the photoactive NH2-TiO2 layer provided not only specific selectivity but also a large surface area as well as an unprecedented photocatalytic activity for the NH2-TiO2/UCNPs-rGO/GCE ECL sensor (TIECLS), which could serve as an identification element for trace phosphoproteins. Under optimal conditions, the TIECLS achieved a relatively low detection limit of 9.2 × 10-5 mg/mL (S/N = 3). Practical application of this TIECLS was carried out in different food samples with satisfying results, which were validated by laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS).
Jianping Guo, Shijie Li, Shuo Wang, Junping Wang. Determination of Trace Phosphoprotein in Food Based on Fluorescent Probe-Triggered Target-Induced Quench by Electrochemiluminescence. Journal of agricultural and food chemistry. 2020 Nov 11;68(45):12738-12748
PMID: 33107726
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