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Fucose-containing oligosaccharides (FCOs) have important applications in the food, medicine, and cosmetics industries owing to their unique biological activities. The degradation of microbial fucose-containing exopolysaccharide (FcEPS) is a promising strategy for obtaining FCOs, and bacteriophage-borne glycanase is a useful tool for degrading FcEPS. Here, we aimed to obtain FCOs using bacteriophage-borne glycanase to depolymerize FcEPS from Enterobacter sakazakii. The FcEPS was mainly composed of l-fucose (42.72 %), d-galactose (20.59 %), and d-glucose (21.81 %). Based on the results of nuclear magnetic resonance and mass spectrometry, the obtained FCOs were disaccharide fragments with backbones of β-d-Glcp-(1→4)-β-l-Fucp and α-d-Galp-(1→3)-β-l-Fucp, respectively. So far, few studies of disaccharides prepared from FcEPS have been reported. This study demonstrated that the FcEPS of E. sakazakii was a reliable fucose-containing disaccharide source and that bacteriophage-borne glycanase was an effective degradation tool for obtaining FCOs fragments from FcEPS. Copyright © 2020 Elsevier Ltd. All rights reserved.

Citation

Mengshi Xiao, Xiaodan Fu, Xinyi Wei, Yongzhou Chi, Wei Gao, Ying Yu, Zhemin Liu, Changliang Zhu, Haijin Mou. Structural characterization of fucose-containing disaccharides prepared from exopolysaccharides of Enterobacter sakazakii. Carbohydrate polymers. 2021 Jan 15;252:117139

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PMID: 33183598

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