Xiaoqi Li, Xiaofan Liu, Zhenzhen Jia, Tian Wang, Hongyan Zhang
Chemosphere 2021 JanVitellogenin (VTG) is typically produced by females but when present in males can indicate contamination with estrogenic endocrine-disrupting chemicals (EDCs). Here we used primary hepatocytes isolated from male crucian carp uncontaminated with estrogenic EDCs as a culture model. Nine EDCs were used for validation, and an enzyme-linked immunosorbent assay (ELISA) was used to quantify VTG production by primary hepatocytes. The lower limit of detection of 17β-E2, DES, and HES using the method was 10-12 M. Fresh pork was roasted, and non-hydrolytic acetonitrile-vortexing was used to extract potential estrogenic EDCs. The extracted substances were separated by thin-layer chromatography (TLC), and dimethyl terephthalate present in roast pork was found to induce high VTG concentrations. Therefore, the detection of VTG by ELISA provides a sensitive and reliable method for detecting known and unknown estrogenic EDCs in food products. Copyright © 2020 Elsevier Ltd. All rights reserved.
Xiaoqi Li, Xiaofan Liu, Zhenzhen Jia, Tian Wang, Hongyan Zhang. Screening of estrogenic endocrine-disrupting chemicals in meat products based on the detection of vitellogenin by enzyme-linked immunosorbent assay. Chemosphere. 2021 Jan;263:128251
PMID: 33297196
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