Correlation Engine 2.0
Clear Search sequence regions


  • Flp1 (5)
  • Irc20 (4)
  • plasmid (10)
  • protein levels (1)
  • regulates (1)
  • ubiquitin (2)
  • yeast (2)
  • Sizes of these terms reflect their relevance to your search.

    The endogenous yeast 2-μm plasmid while innocuous to the host, needs to be properly regulated to avoid a toxic increase in copy number. The plasmid copy number control system is under the control of the plasmid encoded recombinase, Flp1. In case of a drop in 2-μm plasmid levels due to rare plasmid mis-segregation events, the Flp1 recombinase together with the cell's homologous recombination machinery, produce multiple copies of the 2-μm plasmid that are spooled during DNA replication. The 2-μm plasmid copy number is tightly regulated by controlled expression of Flp1 as well as its ubiquitin and SUMO modification. Here, we identify a novel regulator of the 2-μm plasmid, the ATPase, ubiquitin ligase, Irc20. Irc20 was initially identified as a homologous recombination regulator, and here we uncover a new role for Irc20 in maintaining the 2-μm plasmid copy number and segregation through regulating Flp1 protein levels in the cell. Copyright © 2020 Jalal, Chalissery and Hassan.

    Citation

    Deena Jalal, Jisha Chalissery, Ahmed H Hassan. Irc20 Regulates the Yeast Endogenous 2-μm Plasmid Levels by Controlling Flp1. Frontiers in molecular biosciences. 2020;7:221


    PMID: 33330615

    View Full Text