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Label-free, three-dimensional (3D) quantitative observations of on-chip vasculogenesis were achieved using optical diffraction tomography. Exploiting 3D refractive index maps as an intrinsic imaging contrast, the vascular structures, multicellular activities, and subcellular organelles of endothelial cells were imaged and analysed throughout vasculogenesis to characterise mature vascular networks without exogenous labelling.


Chungha Lee, Seunggyu Kim, Herve Hugonnet, Moosung Lee, Weisun Park, Jessie S Jeon, YongKeun Park. Label-free three-dimensional observations and quantitative characterisation of on-chip vasculogenesis using optical diffraction tomography. Lab on a chip. 2021 Feb 09;21(3):494-501

PMID: 33492325

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