Correlation Engine 2.0
Clear Search sequence regions


  • CDKN1A (1)
  • chicken (5)
  • control group (1)
  • CX3CL1 (1)
  • diet (2)
  • gga miR- 17 (1)
  • IL11Ra (1)
  • lymphoid tissue (1)
  • mirna (3)
  • mirnas (9)
  • PIGR (1)
  • receptor (1)
  • SIKE1 (1)
  • spleen (4)
  • target genes (5)
  • Sizes of these terms reflect their relevance to your search.

    For investigating the effects of stress on the immune response of chickens, we established a corticosterone (CORT)-induced stress model by exogenous intake of CORT. Control group was fed with a basal diet and the stress model group was fed with a 30 mg/Kg CORT-treated diet in ad libitum conditions for 7 days. Then, we used RNA-seq technology to identify the expression pattern of miRNAs, target genes, and relevant pathways in chicken spleen. Results showed that 71 differentially expressed miRNAs (DEMs) were determined, 9 of which were significantly differentially expressed miRNAs (SDEMs), and 241 target genes of DEMs were predicted. GO annotation and KEGG pathway analysis were carried out to understand the role of the DEMs. Out of 287 significantly enriched GO terms, 37 were stress- or immune-related, such as response to light stimulus, detection of oxidative stress, and immune response in mucosal-associated lymphoid tissue. Out of 85 KEGG pathways, 8 were related to stress or immunity, such as cytokine-cytokine receptor interaction, JAK-STAT signaling pathway, and RLR signaling pathway. We then constructed the interaction networks between target genes from immune-related pathways and their DEMs. The analysis results suggested that some DEMs (gga-miR-17 family, gga-miR-15/16 family, gga-miR-2954 and gga-miR-34b-5p) and target genes (SIKE1, CX3CL1, IL11Ra, PIGR, and CDKN1A) were core miRNAs and genes. This study revealed the dynamic miRNA transcriptome, target genes and related pathways in chicken spleen under CORT-induced stress model, which provided a basis for studying the molecular mechanism of stress affecting immune function. Copyright © 2021 Elsevier Ltd. All rights reserved.

    Citation

    Aru Su, Yanting Zhou, Yujie Guo, Xiuling Yang, Yanhua Zhang, Wenting Li, Yadong Tian, Kui Li, Guirong Sun, Ruirui Jiang, Ruili Han, Xiangtao Kang, Fengbin Yan. Identification and expression analysis of MicroRNAs in chicken spleen in a corticosterone-induced stress model. Research in veterinary science. 2021 May;136:287-296

    Expand section icon Mesh Tags

    Expand section icon Substances


    PMID: 33740563

    View Full Text