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This protocol outlines a reliable and versatile approach to isolate stromal vascular fraction cells from different adipose tissues across human and mouse species. A number of downstream applications can then be performed to gain an appreciation of the functional activity of unique adipose tissue-resident cell populations. For complete details on the use and execution of this protocol, please refer to Macdougall et al. (2018). © 2021 The Author(s).

Citation

Robert Hearnden, Balraj Sandhar, Vishal Vyas, M Paula Longhi. Isolation of stromal vascular fraction cell suspensions from mouse and human adipose tissues for downstream applications. STAR protocols. 2021 Jun 18;2(2):100422

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PMID: 33870227

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