In this issue of Cell Chemical Biology, Hentrich et al. (2021) describe the application of the SpyCatcher technology to antibody discovery and validation. Fab-SpyTag fusion proteins can be expressed in the periplasm of protease-deficient bacteria and coupled in a modular manner to a variety of SpyCatcher-tagged proteins for improved assay performance. Copyright © 2021 Elsevier Ltd. All rights reserved.
Ashley K Grahn, Christina J Miller, Brian K Kay. Plug and play with recombinant antibody fragments. Cell chemical biology. 2021 Jun 17;28(6):745-747
PMID: 34143957
View Full Text