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In this issue of Cell Chemical Biology, Hentrich et al. (2021) describe the application of the SpyCatcher technology to antibody discovery and validation. Fab-SpyTag fusion proteins can be expressed in the periplasm of protease-deficient bacteria and coupled in a modular manner to a variety of SpyCatcher-tagged proteins for improved assay performance. Copyright © 2021 Elsevier Ltd. All rights reserved.

Citation

Ashley K Grahn, Christina J Miller, Brian K Kay. Plug and play with recombinant antibody fragments. Cell chemical biology. 2021 Jun 17;28(6):745-747

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PMID: 34143957

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