TTK Isoforms Interact with Two Regions of the Mep-1 Protein of Drosophila melanogaster.

K Y Khalisova, I S Osadchiy, P G Georgiev, O G Maksimenko

Doklady. Biochemistry and biophysics 2021 May

filter terms:

The Drosophila TTK protein is involved in the processes of cell differentiation and is represented by two isoforms, TTK69 and TTK88, which have a common N-terminal BTB domain and different C-terminal sequences. Earlier, it was shown that TTK69 represses the activity of enhancers and promoters by recruiting a conserved among higher eukaryotes NURD complex to chromatin. The Mep-1 protein was found in the NURD-complex of Drosophila, and this protein can interact with the C-terminal region of TTK69. In the present study, using the yeast two-hybrid system, we mapped the interacting regions of the TTK and Mep-1 proteins. We identified regions in the unique C-terminal regions of TTK isoforms that can interact simultaneously with two regions of the Mep-1 protein. The results show that, despite the low homology of the C-terminal regions, the TTK isoform retains the ability to interact with two conserved regions of the Mep-1 protein, which suggests the functional significance of this interaction.

Citation

K Y Khalisova, I S Osadchiy, P G Georgiev, O G Maksimenko. TTK Isoforms Interact with Two Regions of the Mep-1 Protein of Drosophila melanogaster. Doklady. Biochemistry and biophysics. 2021 May;498(1):177-179