Up-regulation of transporters of the MRP family by drugs and toxins.

D Schrenk, P R Baus, N Ermel, C Klein, B Vorderstemann, H M Kauffmann

Food Chemistry & Environmental Toxicology, University of Kaiserslautern, Erwin Schrödinger Str. 52, D-67663, Kaiserslautern, Germany. schrenk@rhrk.uni-kl.de

Toxicology letters 2001 Mar 31

filter terms:

Expression of a variety of ABC efflux pumps including certain conjugate transporters of the multidrug resistance protein (MRP) subfamily is inducible in primate and rodent tissues, and in a variety of cell lines and primary cells in culture. In human cell lines (HepG2, MCF-7), we studied the inducibility of MRPs 1-5. Similar to the rat mrp2 gene, human mrp2 is inducible by the chemical carcinogen 2-AAF, the chemotherapeutic drug cisplatin and the barbiturate phenobarbital, as demonstrated in Northern and Western Blots. Furthermore, the antibiotic rifampicin was identified as MRP2 inducer in HepG2 cells. MRP1 and 4 mRNAs being expressed in human liver at a very low level could not be detected in HepG2 cells after treatment with various agents. However, MRP3 and 5 mRNAs were detected in addition to MRP2 and their expression was found to be increased by 2-AAF, cisplatin and rifampicin. MRP1 expression was studied in MCF-7 cells where the chemotherapeutic drug vinblastine and tert-butyl hydroquinone but not the MRP2 inducing agents described above acted as inducers.

Citation

D Schrenk, P R Baus, N Ermel, C Klein, B Vorderstemann, H M Kauffmann. Up-regulation of transporters of the MRP family by drugs and toxins. Toxicology letters. 2001 Mar 31;120(1-3):51-7