Interaction of bepridil with the cardiac troponin C/troponin I complex.

E Abusamhadneh, M B Abbott, A Dvoretsky, N Finley, S Sasi, P R Rosevear

Department of Molecular Genetics, Biochemistry, and Microbiology, University of Cincinnati, College of Medicine, 231 Albert B. Sabin Way, Cincinnati, OH 45267, USA.

FEBS letters 2001 Sep 28

filter terms:

We have investigated the binding of bepridil to calcium-saturated cardiac troponin C in a cardiac troponin C/troponin I complex. Nuclear magnetic resonance spectroscopy and [(15)N,(2)H]cardiac troponin C permitted the mapping of bepridil-induced amide proton chemical shifts. A single bepridil-binding site in the regulatory domain was found with an affinity constant of approximately 140 microM(-1). In the presence of cardiac troponin I, bepridil binding to the C domain of cardiac troponin C was not detected. The pattern of bepridil-induced chemical shifts is consistent with stabilization of more open regulatory domain conformational states. A similar pattern of chemical shift perturbations was observed for interaction of the troponin I cardiac-specific amino-terminus with the cardiac troponin C regulatory domain. These results suggest that both bepridil and the cardiac-specific amino-terminus may mediate an increase in calcium affinity by interacting with and stabilizing open regulatory domain conformations. Chemical shift mapping suggests a possible role for inactive calcium-binding site I in the modulation of calcium affinity.

Citation

E Abusamhadneh, M B Abbott, A Dvoretsky, N Finley, S Sasi, P R Rosevear. Interaction of bepridil with the cardiac troponin C/troponin I complex. FEBS letters. 2001 Sep 28;506(1):51-4