Characterization of cloned mouse Na+/taurocholate cotransporting polypeptide by transient expression in COS-7 cells.

Tohru Saeki, Noriki Takahashi, Ryuhei Kanamoto, Kimikazu Iwami

Department of Biological Resource Chemistry, Faculty of Agriculture, Kyoto Prefectural University, Japan. tsaeki@kpu.ac.jp

Bioscience, biotechnology, and biochemistry 2002 May

filter terms:

The mouse Na+/taurocholate cotransporting polypeptide transiently expressed in COS-7 cells caused sodium-dependent uptake of [3H]taurocholic acid with Km and Vmax values of 18 microM and 102 pmol/mg protein/min, respectively. This Km value is comparable to that for rat NTCP and higher than that for human NTCP. Substrate specificity was evaluated by measuring inhibitory effects of unlabeled bile acids on [3H]taurocholic acid transport.

Citation

Tohru Saeki, Noriki Takahashi, Ryuhei Kanamoto, Kimikazu Iwami. Characterization of cloned mouse Na+/taurocholate cotransporting polypeptide by transient expression in COS-7 cells. Bioscience, biotechnology, and biochemistry. 2002 May;66(5):1116-8