Synthesis of a rabbit polyclonal antibody to the human sodium-dependent multivitamin transporter.

Jacob B Griffin, J Steven Stanley, Janos Zempleni

Department of Nutritional Sciences and Dietetics, University of Nebraska at Lincoln, 316 Ruth Leverton Hall, Lincoln, NE 68583-0806, USA.

International journal for vitamin and nutrition research. Internationale Zeitschrift für Vitamin- und Ernährungsforschung. Journal international de vitaminologie et de nutrition 2002 Jul

filter terms:

In mammalian cells, biotin is covalently attached to carboxylases and histones and is required for cell proliferation and function. Cellular uptake of biotin (as well as pantothenic acid and lipoic acid) is mediated by the sodium-dependent multivitamin transporter, SMVT. Studies of cellular biotin home ostasis have been hampered by the lack of an antibody to SMVT. Here, we describe the synthesis of a rabbit polyclonal antibody to human SMVT. Using this antibody, SMVT has been identified in human peripheral blood mononuclear cells, Caco-2 cells, and HepG2 cells. Moreover, we observed that cells respond to proliferation with increased synthesis of SMVT.

Citation

Jacob B Griffin, J Steven Stanley, Janos Zempleni. Synthesis of a rabbit polyclonal antibody to the human sodium-dependent multivitamin transporter. International journal for vitamin and nutrition research. Internationale Zeitschrift für Vitamin- und Ernährungsforschung. Journal international de vitaminologie et de nutrition. 2002 Jul;72(4):195-8