Involvement of the system L amino acid transporter on uptake of S-nitroso-L-cysteine, an endogenous S-nitrosothiol, in PC12 cells.

Takanori Nemoto, Naoko Shimma, Syunji Horie, Takeshi Saito, Yasunobu Okuma, Yasuyuki Nomura, Toshihiko Murayama

Laboratory of Chemical Pharmacology, Graduate School of Pharmaceutical Sciences, Chiba University, 263-8522, Chiba, Japan.

European journal of pharmacology 2003 Jan 1

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Previously, we proposed that S-nitroso-L-cysteine, an endogenous S-nitrosothiol, was incorporated via the system L-like amino acid transporter(s) in rat brain slices. In this study, we investigated the effect of S-nitroso-L-cysteine on L-[3H]leucine uptake in PC12 cells (a neuronal cell line). L-[3H]Leucine uptake in PC12 cells was Na(+) independent and significantly inhibited by an inhibitor of system L and by L-phenylalanine, L-cysteine, L-methionine and L-leucine at 1 mM. The effects of L-alanine, L-serine and L-threonine were limited. S-Nitroso-L-cysteine, but not other nitric oxide compounds, inhibited L-[3H]leucine uptake, and this inhibitory effect was eliminated by washing with buffer. System L is composed of the 4F2 light chains (LAT1 or LAT2) and the heavy chain, and the transcripts of these components were detected in RNA from PC12 cells. These findings suggest that S-nitroso-L-cysteine is incorporated via the system L amino acid transporter and thus regulates cell responses in PC12 cells.

Citation

Takanori Nemoto, Naoko Shimma, Syunji Horie, Takeshi Saito, Yasunobu Okuma, Yasuyuki Nomura, Toshihiko Murayama. Involvement of the system L amino acid transporter on uptake of S-nitroso-L-cysteine, an endogenous S-nitrosothiol, in PC12 cells. European journal of pharmacology. 2003 Jan 1;458(1-2):17-24