Kinetic mechanism of asparagine synthetase from Vibrio cholerae.

Vicente Fresquet, James B Thoden, Hazel M Holden, Frank M Raushel

Department of Chemistry, Texas A&M University, PO Box 30012, College Station, TX 77843-3012, USA.

Bioorganic chemistry 2004 Apr

filter terms:

Asparagine synthetase B (AsnB) catalyzes the formation of asparagine in an ATP-dependent reaction using glutamine or ammonia as a nitrogen source. To obtain a better understanding of the catalytic mechanism of this enzyme, we report the cloning, expression, and kinetic analysis of the glutamine- and ammonia-dependent activities of AsnB from Vibrio cholerae. Initial velocity, product inhibition, and dead-end inhibition studies were utilized in the construction of a model for the kinetic mechanism of the ammonia- and glutamine-dependent activities. The reaction sequence begins with the ordered addition of ATP and aspartate. Pyrophosphate is released, followed by the addition of ammonia and the release of asparagine and AMP. Glutamine is simultaneously hydrolyzed at a second site and the ammonia intermediate diffuses through an interdomain protein tunnel from the site of production to the site of utilization. The data were also consistent with the dead-end binding of asparagine to the glutamine binding site and PP(i) with free enzyme. The rate of hydrolysis of glutamine is largely independent of the activation of aspartate and thus the reaction rates at the two active sites are essentially uncoupled from one another.

Citation

Vicente Fresquet, James B Thoden, Hazel M Holden, Frank M Raushel. Kinetic mechanism of asparagine synthetase from Vibrio cholerae. Bioorganic chemistry. 2004 Apr;32(2):63-75