Acetazolamide inhibits aquaporin-1 protein expression and angiogenesis.

Yang Xiang, Bing Ma, Tao Li, Jun-Wei Gao, He-Ming Yu, Xue-Jun Li

Department of Pharmacology, School of Basic Medical Sciences, Peking University, Beijing 100083, China.

Acta pharmacologica Sinica 2004 Jun

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To study effects of acetazolamide on aquaporin-1 (AQP(1)) protein expression and angiogenesis. Establishing Lewis-lung-carcinoma model, the localization of AQP(1) in tumor tissues was investigated by immunohistochemical methods; The biological activity of acetazolamide was detected by endothelial cells proliferation test (MTT) assay and chorioallantoic membrane (CAM) vascular inhibition test. Immunohistochemical localization of AQP(1) in mice tumor was labeled in capillaries, post capillary venules endo thelial cells. After being treated with acetazolamide, the number of capillaries and post capillary venules was significantly decreased in tumor tissue. Acetazolamide showed significant inhibitory effect on angiogenesis in CAM and endothelial cell proliferation. Acetazolamide might be identified and developed as one of potential lead compounds for a new therapeutic intervention in inhibiting cancer angiogenesis.

Citation

Yang Xiang, Bing Ma, Tao Li, Jun-Wei Gao, He-Ming Yu, Xue-Jun Li. Acetazolamide inhibits aquaporin-1 protein expression and angiogenesis. Acta pharmacologica Sinica. 2004 Jun;25(6):812-6