Harish Chandra Goel, Damodar Gupta, Shobha Gupta, A P Garg, Madhu Bala
Radiation Biology Division, Institute of Nuclear Medicine and Allied Sciences, Brig. S. K. Mazumdar Road, Delhi-110 054, India. goelharish@hotmail.com
The Journal of pharmacy and pharmacology 2005 JanThe whole extract of the fresh berries of Hippophae rhamnoides L. (RH-3), which has been reported to provide protection to whole mice, various tissues, cells and cell organelles against lethal irradiation, was further investigated for its effects on mitochondria isolated from mouse liver. Superoxide anion, reduced (GSH) and oxidized glutathione (GSSG) levels, NADH-ubiquinone oxidoreductase (complex I), NADH-cytochrome c oxidoreductase (complex I/II), succinate-cytochrome c oxidoreductase (complex II/III), mitochondrial membrane potential (MMP), lipid peroxidation (LPx) and protein oxidation (PO) were determined for RH-3-mediated radioprotective manifestation. Pre-irradiation treatment of mice with RH-3 (30 mg kg(-1,) i. p.; single dose; -30 min) significantly inhibited the radiation-induced increase in superoxide anions, GSSG, thiobarbituric acid reactive substances (TBARS), complex I, complex I/III activity and MMP maximally at 4 h (P < 0.05). This treatment inhibited the oxidation of proteins (P < 0.05) at all the time periods studied here. This study suggests that pre-irradiation treatment of mice with RH-3 protects the functional integrity of mitochondria from radiation-induced oxidative stress.
Harish Chandra Goel, Damodar Gupta, Shobha Gupta, A P Garg, Madhu Bala. Protection of mitochondrial system by Hippophae rhamnoides L. against radiation-induced oxidative damage in mice. The Journal of pharmacy and pharmacology. 2005 Jan;57(1):135-43
PMID: 15639001
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