Zhigang Xie, Ning Guo, Ming Yu, Meiru Hu, Beifen Shen
Department of Molecular Immunology, Beijing Institute of Basic Medical Sciences, Taiping Road 27, Beijing 100850, China.
Journal of immunological methods 2005 JanBispecific antibodies (BsAbs) have been considered as potential therapeutics for cancer. A major obstacle in the development of BsAb has been the difficulty in producing a heterodimer with two different arms and in sufficient quantity for clinical application by the traditional methods. We describe a new format of BsAb that consists of two single-chain variable fragment of antibodies (scFvs), one for human epidermal growth factor receptor 2 (HER2)/neu and the other for CD16, heterodimerized by a "knobs-into-holes" device from the CH3 domains of the human IgG1 Fc fragment. The two chains were functionally expressed in CHO cells and assembled into heterodimers with dual antigen-binding specificity. Compared with other types of engineered BsAbs expressed in mammalian cells, the yield of this BsAb was relatively high (12-14 mg/l). In vitro experiments demonstrated that the BsAb was able to recruit human peripheral blood mononuclear cells (PBMC) to kill SK-BR-3 cells more effectively than the commercial anti-HER2/neu antibody Herceptin (Roche, Shanghai). This new format of BsAb possesses properties that support its potential as a new antitumor agent.
Zhigang Xie, Ning Guo, Ming Yu, Meiru Hu, Beifen Shen. A new format of bispecific antibody: highly efficient heterodimerization, expression and tumor cell lysis. Journal of immunological methods. 2005 Jan;296(1-2):95-101
PMID: 15680154
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