High-mannose-type glycan modifications of dihydrofolate reductase using glycan-methotrexate conjugates.

Kiichiro Totani, Ichiro Matsuo, Yoshito Ihara, Yukishige Ito

RIKEN (The Institute of Physical and Chemical Research), 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan.

Bioorganic & medicinal chemistry 2006 Aug 1

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Various high-mannose-type glycan modifications of dihydrofolate reductase (DHFR) were achieved by ligand-based approach using glycan-methotrexate (MTX) conjugates as tight binding glycan bearing ligands for DHFR. The resulting glycan-MTX conjugates and the corresponding artificial glycoproteins could be useful as oligosaccharide- and glycoprotein-probes to perform quantitative analysis of glycan recognizing protein such as lectins, glycosyltransferases or glycosidases. Moreover, artificial glycoproteins having two different high-mannose-type glycans were developed for the first time by a combination of two different types of glycan modification strategies.

Citation

Kiichiro Totani, Ichiro Matsuo, Yoshito Ihara, Yukishige Ito. High-mannose-type glycan modifications of dihydrofolate reductase using glycan-methotrexate conjugates. Bioorganic & medicinal chemistry. 2006 Aug 1;14(15):5220-9