Glucosamine sulfate suppresses the expressions of urokinase plasminogen activator and inhibitor and gelatinases during the early stage of osteoarthritis.

Shu-Chen Chu, Shun-Fa Yang, Ko-Huang Lue, Yih-Shou Hsieh, Ching-Yi Lee, Ming-Chih Chou, Ko-Hsiu Lu

Department of Food Science, Central Taiwan University of Science and Technology, Taichung 406, Taiwan.

Clinica chimica acta; international journal of clinical chemistry 2006 Oct

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Glucosamine sulfate may have an ex vivo inhibitory effect on the plasminogen activator (PA)/plasmin system and gelatinases expression during the early development of osteoarthritis (OA). We compared the levels of urokinase-type PA (u-PA), PA inhibitor-1 (PAI-1) and gelatinases (matrix metalloproteinase-2 and -9 [MMP-2 and -9]) in a series of chondral, meniscal, and synovial cultures of early OA after treatment with or without glucosamine sulfate. Gelatin zymography revealed that glucosamine sulfate could suppress MMP-2 secretion in chondral, meniscal and synovial cultures and also decrease MMP-9 production in synovial and meniscal cultures. ELISA data also showed the suppressive effects of glucosamine sulfate on u-PA and PAI-1 production in synovial cultures at 48 h. Our data suggest that one of the therapeutic effects of glucosamine sulfate is to down-regulate the expressions of u-PA, PAI-1, MMP-2 and MMP-9 that underlie the destruction of articular cartilage in the early stage of OA, and therefore to delay the joint failure.

Citation

Shu-Chen Chu, Shun-Fa Yang, Ko-Huang Lue, Yih-Shou Hsieh, Ching-Yi Lee, Ming-Chih Chou, Ko-Hsiu Lu. Glucosamine sulfate suppresses the expressions of urokinase plasminogen activator and inhibitor and gelatinases during the early stage of osteoarthritis. Clinica chimica acta; international journal of clinical chemistry. 2006 Oct;372(1-2):167-72