Potentiation by neuropeptide Y of histamine H1 receptor-mediated contraction in rat blood vessels.

Honglan Piao, Shingo Nagai, Tatsuru Tsurumaki, Takeshi Niki, Hiroshi Higuchi

Division of Pharmacology, Department of Molecular genetics and Signal Transduction Research, Course for Molecular and Cellular Medicine, Niigata University Graduate School of Medical and Dental Sciences, Japan.

Vascular pharmacology 2007 Apr

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Histamine-induced contraction and its potentiation by neuropeptide Y were investigated in rat blood vessels. Rat arteries and veins constricted with single concentrations of histamine dose-dependently (0.1-100 microM). This histamine-induced contraction immediately desensitized. Histamine H1 receptor antagonists, 1 microM mepyramine and 1 microM diphenhydramine, abolished this transient contraction completely, whereas cimetidine, phentolamine, reserpine and tetrodotoxin failed to inhibit the contraction. Histamine H1 receptor mRNA level by reverse transcription-polymerase chain reaction was quite parallel to histamine H1 receptor-mediated contraction, indicating that the contraction is mediated through histamine H1 receptors in the smooth muscle. Neuropeptide Y (10 nM in arteries and 3 nM in veins, respectively) significantly potentiated histamine H1 receptor-mediated contraction via neuropeptide Y1 receptors in most of rat blood vessels. Since the phospholipase C inhibitors, neomycin (1 mM) and 2-nitro-4-carboxyphenyl-N, N-diphenylcarbamate (NCDC, 10 microM), respectively, specifically abolished the potentiation, the potentiation by neuropeptide Y may depend on activation of phospholipase C.

Citation

Honglan Piao, Shingo Nagai, Tatsuru Tsurumaki, Takeshi Niki, Hiroshi Higuchi. Potentiation by neuropeptide Y of histamine H1 receptor-mediated contraction in rat blood vessels. Vascular pharmacology. 2007 Apr;46(4):260-70