Protection of cells from oxidative stress by microsomal glutathione transferase 1.

Atchasai Siritantikorn, Katarina Johansson, Karin Ahlen, Rosanna Rinaldi, Tuangporn Suthiphongchai, Prapon Wilairat, Ralf Morgenstern

Department of Biochemistry, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.

Biochemical and biophysical research communications 2007 Apr 6

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Rat liver microsomal glutathione transferase 1 (MGST1) is a membrane-bound enzyme that displays both glutathione transferase and glutathione peroxidase activities. We hypothesized that physiologically relevant levels of MGST1 is able to protect cells from oxidative damage by lowering intracellular hydroperoxide levels. Such a role of MGST1 was studied in human MCF7 cell line transfected with rat liver mgst1 (sense cell) and with antisense mgst1 (antisense cell). Cytotoxicities of two hydroperoxides (cumene hydroperoxide (CuOOH) and hydrogen peroxide) were determined in both cell types using short-term and long-term cytotoxicity assays. MGST1 significantly protected against CuOOH and against hydrogen peroxide (although less pronounced and only in short-term tests). These results demonstrate that MGST1 can protect cells from both lipophilic and hydrophilic hydroperoxides, of which only the former is a substrate. After CuOOH exposure MGST1 significantly lowered intracellular ROS as determined by FACS analysis.

Citation

Atchasai Siritantikorn, Katarina Johansson, Karin Ahlen, Rosanna Rinaldi, Tuangporn Suthiphongchai, Prapon Wilairat, Ralf Morgenstern. Protection of cells from oxidative stress by microsomal glutathione transferase 1. Biochemical and biophysical research communications. 2007 Apr 6;355(2):592-6